Acetyl-Coenzyme A Synthetase, Cytoplasmic (Acetyl-CoA Synthetase, AceCS1, AceCS, ACS, ACSA, Acyl-CoA Synthetase Short-chain Family Member 2, ACAS2, ACSS2, Acetate-CoA Ligase, Acyl-activating Enzyme, dJ1161H23.1, DKFZp762G026), Clo
Artikelnummer:
USB-A0526-48
Hersteller Artikelnummer:
A0526-48
Alternativnummer:
USB-A0526-48-20,USB-A0526-48-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IP, WB
Immunogen:
Synthetic peptide corresponding to the sequence of human cytoplasmic acetyl-CoA synthetase (AceCS1).
Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA (1, 2). Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1, 2). Studies suggest that this enzyme is regulated by sterol regulatory element-binding proteins (2). It was also shown that this enzyme is acetylated by protein acetyltransferases in cells (3, 4). SIRT1 deacetylates this enzyme at Lys661 resulting in its activation and the increase of fatty acid synthesis (3, 4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
