Adenosine A2a Receptor, Western Blot Positive Control (A2aR, ADORA2A, RDC8)
Artikelnummer:
USB-A0869-56H
Hersteller Artikelnummer:
A0869-56H
Alternativnummer:
USB-A0869-56H-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
WB
The purine nucleoside adenosine modulates diverse physiological functions including induction of sedation, vasodilatation, suppression of cardiac rate and contractility, neurotransmitter release, inhibition of platelet aggregation and lipolysis. Adenosine released from cells interacts with membrane receptors (adenosine receptors, ARs). Based upon, biochemical and pharmacological criteria, ARs have been classified into A1, A2a, A2b, and A3. The high affinity receptor A1 inhibits adenyl cyclase, whereas low affinity receptor A2a stimulates the cyclase via G proteins. A2a receptor is believed to cause vasorelaxation in coronary artery. Various, ARs and their subtypes have recently been cloned from several species. ARs belong to the superfamily of G-protein coupled receptors and predicted to contain 7 transmembrane domains. The N-termini are predicted to be extracellular and the C-termini cytoplasmic. There is an overall 30% homology among the four ARs. ARs are distributed throughout the body. Gene location of various ARs: A1 (Chromosome 1, q.31.3-32.2), A2a (Chromosome 22), A2b (Chromosome 17, p.11.2-12), A3 Chromos.1). Source: A2aR protein, from bovine brain. Applications: Suitable for use in Western Blot. Not suitable for ELISA or other applications where native protein is required. Other applications not tested. Recommended Dilution: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
