Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cells cellular physiology. Adipsin is serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259-aa, rat 263 aa, human 253 aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipocyte is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml using ECL. Human Adipsin is ~22kD. ELISA: 1:10,000-1:50,000 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified by salt fractionation, Ion-Exchange and Protein A affinity chromatography
Formulierung:
Supplied as a liquid in 100mM Tris, pH 7.5, 0.05% sodium azide
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