Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28), Rabbit

Produktübersicht

• Artikelname: Adiponectin (Acrp30, Adipocyte Complement-related Protein of 30kD, AdipoQ, apM1, Gelatin Binding Protein 28, GPB28), Rabbit
• Artikelnummer: USB-A0891
• Hersteller Artikelnummer: A0891
• Alternativnummer: USB-A0891-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Recombinant Murine Adiponectin produced in HEK293 (Human embryonic kidney cell line).

Produktbeschreibung

Adiponectin (also called ACRP30 and GBP28) is a protein exclusively secreted from adipose tissue. Full-length adiponectin was expressed in HEK293 cells stably transfected with pcdm-F vector encoding FLAG epitope-tagged mouse adiponectin. Recombinant adiponectin was purified using anti-FLAG M2 affinity gel (Sigma-Aldrich) and was eluted with FLAG peptide. FLAG peptides were removed by an extensive wash with an excessive volume of saline. Highly sensitive matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass-spectrometric analysis confirmed that FLAG peptide was not detectable in the protein solution. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Indirect ELISA: >1:100,000 using 25ng of antigen coated per well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• UniProt: Q60994
• Reinheit: Purified by immunoaffinity chromatography.
• Formulierung: Supplied as a lyophilized powder in PBS, pH 7.2