Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.
ARF1 (ADP-ribosylation factor 1) is a member of the ARF family of small G proteins (guanine nucleotide-binding proteins). It is a component of Golgi-derived non-clathrin coated vesicles. Arf and other related small GTPases are an important part of the membrane traffic with both exocytic and endocytic pathways. ARF1 helps regulate the binding and releasing of coat proteins on membranes to form transport vesicles. It is also known to play a role in vessicular trafficking of phospholipase D. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin): 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.