Control Peptide for A0906-91 (antiserum) and A0906-91A (affinity purified antibody). The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptor superfamily. They are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs. This stimulation in turn activates adenyl cyclase. Activation of adenyl cyclase yields the second messenger cAMP. B3AR is primarily present in adipocytes The B3AR plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In humans, B3AR, a Trp to Arg replacement has recently been discovered at position 64. This change in the first coding exon has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Chronic stimulation of the B3AR by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle. This expression may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408aa membrane protein (rat/mouse, 400aa). The N-terminus is predicted to be extracellular, the C-terminus intracellular. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilutions: ELISA: Coat with 50-100ng Control Peptide/well Antibody Blocking: 5-10ug per 1ul A0906-91 (antiserum) or per 1ug A0906-91A (affinity purified antibody). Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Mouse related products: A0906-92: Adrenergic Receptor, beta 3, Mouse (B3AR) (Control Peptide) A0906-92A: Adrenergic Receptor, beta 3 (B3AR) Pab Rb xMo A0906-92B: Adrenergic Receptor, beta 3 (B3AR) Pab Rb xMo
Reinheit:
Highly purified
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide
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