Control Peptide for (affinity purified) and (antiserum). Synthetic peptide, 19aa of Human B3AR. ~C-terminus The beta 3-adrenergic receptor (B3AR) is a member of the super-family of G protein-coupled receptors that are characterized by seven putative transmembrane helices connected by hydrophilic loops. The mechanism by which the activated beta ARs transmit the signals across the plasma membrane involves the stimulation of Gs, which in turn activates adenyl cyclase, yielding the second messenger cAMP. (B3AR) is present primarily in adipocytes The beta 3-adrenoceptor plays a significant role in the control of lipolysis and thermogenesis in the brown adipose tissue of rodents and humans. In human beta 3-adrenoceptor, a Trp to Arg replacement has recently been discovered. This change which occurs at position 64, in the first coding exon, has been correlated with increased weight gain, difficulty in losing weight, insulin resistance syndrome, and worsened diabetic situation. Higher percentages of this mutation are observed in Pima Indians (over 30%) and Japanese (20%). Thus, chronic stimulation of the beta 3-adrenergic receptor by highly selective B3AR agonist CL316,243, induces ectopic expression of UCP1 in adipose tissues and skeletal muscle, which may contribute to the potent anti-obesity effect of the beta 3-adrenergic agonist. Human B3AR is a 408 AA membrane protein (rat/mouse, 400 AA). The N-terminus is predicted to be extracellular, while the C-terminus intracellular. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml to coat plates. Antibody Blocking: 5-10ug peptide. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Highly purified.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
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