| High Gel Strength Agarose Combines very high gel strength with low EEO and is a good choice for high speed, high temperature pulsed field gel electrophoresis applications. Applications: Pulsed Field Electrophoresis Immunoelectrophoresis Blotting Assays (Southern, northern) Analytical DNA Gels 1000bp Preparative Electrophoresis For use with all buffer systems Recommended Usage (1X TAE): Concentration Molecular Weight 0.4-2.0% 50-500kb pH (1.5%, Gel): As Reported EEO: 0.135 Loss on Drying: 7.0% Melting Point (1.5%): 86-90C Gel Point (1.5%): 34.5-37.5C Gel Strength (1.5%): 3000g/cm2 Sulfate: 0.10% Ash: 0.25% Clarity: 40 NTU RNase: None Detected DNase: None Detected Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. Functional and Molecular Biology Testing: To Pass Test Comparative assay of different size DNA fragments Southern Blot (125-23,000bp) Background Fluorescence (TAE/TE, EtBr): DNA Binding (TAE, EtBr) Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4C for 1 hour before use. |
