AHCY, Recombinant, Human (Adenosylhomocysteinase, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, SAHH)
Artikelnummer:
USB-A1059-73G
Hersteller Artikelnummer:
A1059-73G
Alternativnummer:
USB-A1059-73G-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Human S-Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD cofactor for each subunit (1 2). It is the only known enzyme to catalyze the breakdown of Sadenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adonosylLmethionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S-adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4 5). AHCY deficiency is one of the causes of hypermethioninemia, a rare disease affecting brain development and muscle strength. Down-regulation of AHCY is also associated with certain forms of cancer, Huntingtons disease and Wilsons disease. Source: Recombinant corresponding to Ser2-Tyr432 from human Adenosylhomocysteinease/AHCY, N-terminal Met, 6-His tag, expressed in E. coli. Molecular Weight: ~49kD Biological Activity: Measured by the hydrolysis of S-adenosyl-L-homocysteine. The specific activity is >350 pmoles/min/ug, as measured under the described conditions. Endotoxin Level: <1EU/ug (LAL method) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
