Atk1, inactive, Recombinant, Human, N-terminal His-Tag, Serine/Threonine Kinase

Produktübersicht

• Artikelname: Atk1, inactive, Recombinant, Human, N-terminal His-Tag, Serine/Threonine Kinase
• Artikelnummer: USB-A1125-01D
• Hersteller Artikelnummer: A1125-01D
• Alternativnummer: USB-A1125-01D-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW 60.2kD, expressed in an Baculovirus infected Sf9 cell expression system. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Source: Baculovirus infected Sf9 cells Specific Activity: 100U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1nmol of Crosstide substrate per minute at pH 7.4 and 30C. Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.1mM ATP. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Produkteigenschaften

• NCBI: 001014432
• UniProt: P31749
• Reinheit: 80% by SDS-PAGE.
• Formulierung: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.