ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase), Rabbit
Artikelnummer:
USB-A1210-02D
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| Artikelname: | ALAS1 (Aminolevulinate delta-Synthase 1, 5-Aminolevulinic Acid Synthase, Delta-ALA Synthetase), Rabbit |
| Artikelnummer: | USB-A1210-02D |
| Hersteller Artikelnummer: | A1210-02D |
| Alternativnummer: | USB-A1210-02D-100 |
| Hersteller: | US Biological |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IHC, WB |
| Immunogen: | Synthetic peptide corresponding to aa480-530 of human ALAS-1. Species sequence homology canine, chicken and primate |
| ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5 phosphate dependent housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HepG2 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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