Recombinant protein corresponding to Gln18-Ser740 (predicted) from human ACE-2 expressed in NS0 cells.
Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml NS0 mouse myeloma cell line and human kidney tissue Immunoprecipitation: 25ug/ml conditioned cell culture medium spiked with recomninant human ACE-2 Immunohistochemistry: 1-25ug/ml on frozen sections of rat kidney and paraffin-embedded sections of hamster lung. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
