A 15aa Peptide sequence near the cytoplasmic C-terminus of human AE4. Control Peptide for A2295-14G (antiserum) and A2295-14G1 (affinity purified) Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300 aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Recently, a new member of AE family, AE4, has been clones from rabbit and human. Human AE4 (945 aa, chromosome 5) is predicted to have 14 TM domains. It is strongly expressed in the kidney. Rabbit and human AE4 share 84% identity. Recognizes human AE4. Sequence homology: Human isoforms 1AE, 2, 3, NBC5 (AB032762, 657 aa) and NBC (AF313465, 990aa, XM_038736, 959aa)-100%. Species sequence homology: rabbit-100% Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer..
Reinheit:
Highly purified
Formulierung:
Supplied as a liquid in PBS, pH 7.2
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