Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (APOBEC3G) is a host cellular protein with a broad antiviral activity on a wide variety of retroviruses including HIV-1, other lentiviruses, and murine leukemia virus (MLV) [2-4]. It inhibits infectivitiy of a wide variety of retroviruses by deaminating deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA. It belongs to the Apobec superfamily of cytidine deaminases [5] and inhibits the infectivity by being packaged into virions. During reverse transcription, it deaminates deoxycytidine (dC) into deoxyuridine (dU) in newly synthesized minus strand DNA, resulting in either G-to-A hypermutation of the viral plus strand DNA or degradation of dU-rich reverse transcripts [3,6-8], Most lentiviruses express an accessory protein called virion infectivity factor (Vif) which blocks the antiviral function of APOBEC3G by preventing its packaging into virions. Vif binds to APOBEC3G and induces its ubiquitination and subsequent degradation by the proteasome [9-13]. It has also been reported that APOBEC3G inhibits the replication of hepatitis B virus (HBV) without inducing G-to-A hypermutation [14]. This suggests that APOBEC3G has a broad antiviral activity not only on retroviruses but also on other viruses through different mechanisms from that on retroviruses. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 10-100ng/ml Western Blot:1-5ug/ml Optimal dilutions to be determined by the researcher. Biological Activity: CEM15 biological activity neutralization assays pending. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[5i2]
Isotyp:
IgG1
Reinheit:
Purified by Protein A/G affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.0.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
