Control Peptide for A3000-75 (antiserum) and A3000-80 (affinity purified) A 17aa synthetic peptide within the cytoplasmic, C-terminal region of human AQP10. Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporin. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. A new member of AQP family, AQP10, has been cloned (1). AQP10 (human 264 aa) is most closely related with AQP3 (53%), AQP9 (52%), and AQP7 (43%). It is abundantly expressed in duodenum and jejunum. AQP10 is not permeable to urea and glycerol. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ul A3000-75 (antiserum) or per 1ug A3000-80 (affinity purified antibody). ELISA: 50-100ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Highly purified
Formulierung:
Supplied as a liquid in PBS, pH 7.2
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