Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates. They are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Arachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of ~27kD each. It is galactose-specific, with strong anti-T-antigen activity. T-antigen is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes. It strongly agglutinates neuraminidase-treated red blood cells. The presence of Ca2+ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies. Carbohydrate Specificity: Terminal b-galactose Inhibitory Carbohydrate: Lactose > b-galactose Activity: <1ug/ml will agglutinate human erythrocytes neuraminidase treatment of cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
120
Formulierung:
Supplied as a liquid in PBS, pH 7.4. Labeled with Biotin.
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