ASAHL, Recombinant, Human (N-Acylsphingosine Amidohydrolase-like)
Artikelnummer:
USB-A3597-42H
Hersteller Artikelnummer:
A3597-42H
Alternativnummer:
USB-A3597-42H-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
The human ASAHL gene encodes N-acylethanolamine-hydrolyzing acid amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2). Recombinant human N-acylethanolamine-hydrolyzing acid amidase (NAAA) encoded by the ASAHL gene (rhASAHL, amino acid residues 1-359, Accession Q02083, variant V151I) was expressed with a C-terminal 10X His tag in a Chinese Hamster Ovary cell line. Calculated molecular mass of 39kD and migrates as a 50kD band in SDS-PAGE under reducing conditions. Fragments of 33 and 2 kD, resulting from proteolytic processing during maturation, are also present. Activity: Measured by its ability to hydrolyze the substrate N-palmitoylethanolamide into palmitate and ethanolamine. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
