ATPase, Ca, Rat, Control Peptide (Plasma Membrane, PMCA)

Produktübersicht

• Artikelname: ATPase, Ca, Rat, Control Peptide (Plasma Membrane, PMCA)
• Artikelnummer: USB-A4000-50H
• Hersteller Artikelnummer: A4000-50H
• Alternativnummer: USB-A4000-50H-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA, WB

Produktbeschreibung

A 20aa Peptide sequence near the N-terminus of mature Rat NCKX2 (1) was selected for antibody production. Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers- Three NCX (NCX1, NCX2, and NCX3) and two in the NCKX family (NCKX1 and NCKX2). NCX shares significant sequence homology (~70%), displays 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX contains a highly basic region in the large hydrophilic, intracellular loop called XIP (Exchange inhibitory peptide, RRLLFYKYVYKRYRAGKQRG 20 aa), that inhibits Na-Ca+ exchange in cardiac sarcolemmal vesicles and in other cells. Little or no sequence identity is found between the NCX and the Ca-pump. However, XIP also inhibits the Ca pumps with more or less same efficiency as C28R2 peptide sequence (LRRGQILWFRGLNRIQTQIRVVKAFRSS, 28 aa) corresponding to the autoinhibitory domain of Ca-pump. Applications: ELISA: 1:10-50K for neat serum and 0.5-1ug/ml for affinity purified. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Chromatographically purified
• Formulierung: Supplied as a liquid in PBS, pH 7.2.