| Azidothymidine is a nucleoside reverse transcriptase inhibitor that, following triphosphorylation by thymidine kinase, potently blocks replication of HIV (EC50 = 3nM) with low cytotoxicity (CC50 >5uM). Displays 100-fold selectivity for HIV reverse transcriptase over DNA polymerase alpha. Suppresses HIV-1 replication and enhances cell viability in HIV-1 infected T cells. Antiretroviral agent. Also decreases CRISPR-mediated homology directed repair (HDR) efficiency. Synonyms: AZT, Zidovudine, Retrovis, Timazid, Retrovir, NSC 602670 CAS No: 30516-87-1 Molecular Formula: C10H13N5O4 Molecular Weight: 267.24 Purity (HPLC): 97% Appearance: White to off-white crystalline solid. Melting Point: 118-120C Solubility: Water (Slightly soluble), alcohol (soluble) Water Content (Karl Fischer): 1% Method for Determining Identity: Proton NMR Spectroscopic Residue on Ignition: 0.25% Optical Rotation (C=10mg/ml in Alcohol): [a]20D=60.5-63.0 Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. |
