Bam HI

Produktübersicht

• Artikelname: Bam HI
• Artikelnummer: USB-B0070
• Hersteller Artikelnummer: B0070
• Alternativnummer: USB-B0070-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G G A T C C-3 3-C C T A G G-5 Source: Bacillus amyloliquefaciens H Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Bam HI required to digest 1ug of lambda DNA-Bsp120I fragments in 1 hour at 37C in 50ul of assay buffer. Supplied With: R1625: Restriction Enzyme Buffer A, 10X: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA (pH 7.9 at 37C). B0070A Reaction Buffer, 10X: Supplied as a liquid in 10mM Tris-HCl, pH 8.0, 5mM MgCl2, 100mM KCl, 0.02% Triton X-100, 0.1mg/ml BSA. Dilute and use at 1X. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4 at 25C, 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Incubation Temp: 37C Enzyme Properties: Methylation Effects: Dam: completely overlap - blocked Dcm, CpG: May overlap- no effect EcoKl, EcoBl: Never overlaps- no effect Stability during Prolonged Incubation: A minimum of 0.5 units of Bam HI is required for complete digestion of 1ug of lambda DNA in 16 hr at 37C. Digestion of Agarose-embedded DNA: A minimum of 5 units of Bam HI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hr. Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI Number of Recognition Sites in DNA: Lambda: 5 PhiX174: 0 pBR322, pUC57, pUC18/19, pTZ19R/U, M13mp18/19: 1 Thermal Inactivation: Bam HI (up to 10 units) is inactivated by incubation at 80C for 20min. Quality Control Assay Data: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours). Ligation/ Recutting Assay: The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Bam HI for 4 hr. Blue/White Cloning Assay: The B/W assay was replaced with LO test after validating experiments showed LO test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.