| The Bcl-2 family consists of a number of evolutionarily conserved proteins containing Bcl-2 homology domains (BH) that regulate apoptosis through control of mitochondrial membrane permeability and release of cytochrome c (1-3). Four BH domains have been identified (BH1-4), which mediate protein interactions. The family can be separated into three groups based upon function and sequence homology: pro-surivival members including Bcl-2, Bcl-xL, Mcl-1, A1, and Bcl-w, pro-apoptotic proteins including Bax, Bak, and Bok, and ''BH3 only proteins Bad, Bik, Bid, Puma, Bim, Bmf, Noxa, and Hrk. Interactions between death-promoting and death-suppressing Bcl-2 family members has led to a rheostat model in which the ratio of pro-apoptotic and anti-apoptotic proteins controls cell fate (4). Thus, pro-survival members exert their behavior by binding to and antagonizing death-promoting members. In general, the ''BH3-only members can bind to and antagonize the pro-survival proteins leading to increased apoptosis (5). While some redundancy of this system likely exists, tissue specificity and transcriptional and post-translational regulation of many of these family members can account for distinct physiological roles. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87 (6). Included antibodies: B0807-02D1 Bcl 2, phosphorylated (Ser70) 20ul W, F H 28kD Rabbit B0807-02D2 Bcl-2, phosphorylated (Thr56) 20ul W H 28kD Rabbit B0807-02D3 Bcl-2 20ul W, IP, IHC H 28kD Rabbit B0807-02D4 Bcl-XL 20ul W, IP, IHC H, M, R, Mk 30kD Rabbit B0807-02D5 Mcl-1 20ul W H 40kD Rabbit B0807-02D6 IgG (HRP) 100ul Goat Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Freeze at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
