BglI

Produktübersicht

• Artikelname: BglI
• Artikelnummer: USB-B1087
• Hersteller Artikelnummer: B1087
• Alternativnummer: USB-B1087-2000,USB-B1087-5X2000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G C C N N N N N G G C-3 3-C G G N N N N N C C G-5 Concentration: 10u/ul Source: Bacillus globigii Buffer: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA Incubate at 37C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 300mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BglI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with BglI, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.3uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Methylation Effects: The hemi-methylated sequence GCCN5GGm5C is cleaved by BglI at a significantly slower rate. Cleavage is blocked when both strands are methylated at positions: 5_ GCCN5GGm5C_3 3_m5CGGN5CC G_5. Overlapping CG methylation may influence DNA cleavage. Stability during Prolonged Incubation: A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 29 PhiX174: 0 M13mp18/19: 1 pBR322: 3 pUC18/19: 2 pUC57: 2 pTZ19R/U: 2 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 1 pACYC184: 2 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.