| Control Peptide for B2553-03G. The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and -II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function. Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and -13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation. BMP1 (EC 3.4.24.1, procollagen C-endopeptidase, procollagen C-proteinase, PCP, mammalian tolloid) was first identified in osteogenic bone extracts. It is an extracellular zinc endopeptidase implicated in a variety of species. BMP1 is expressed at high levels in embryonic maternal deciduum, membranous and endochondral bone, submucosa of intestine, dermis of skin and the mesenchyme of spleen and lung. BMP1 is a shorter spliced variant of mammalian tolloid (mTld) with a 986-aa sequence (chr 8p21), both of which cleave the C-propeptides of type I procollagen during the synthesis of extracellular matrix collagen fibrils. BMP-1 comprises a metalloproteinase domain, 3 CUB domains, and an EGF-like domain, which is located between the second and third CUB domains. It cleaves the C-terminal propeptides of procollagen I, II and III, induces cartilage and bone formation. It shows 7 alternatives spliced forms and ubiquitously expressed. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml to coat plates. Antibody Blocking: 5-10ug peptide. Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
