Bone Morphogenetic Protein 2, Recombinant, Human (BMP2)

Produktübersicht

• Artikelname: Bone Morphogenetic Protein 2, Recombinant, Human (BMP2)
• Artikelnummer: USB-B2553-06A
• Hersteller Artikelnummer: B2553-06A
• Alternativnummer: USB-B2553-06A-2,USB-B2553-06A-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate, ovary, and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 is a 26kD homodimeric protein consisting of two 115 amino acid polypeptide chains. Recombinant protein corresponding to human Bone Morphogenetic Protein-2, expressed in E. coli. Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. RReconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 26
• UniProt: P12643
• Reinheit: 98% (HPLC and SDS-PAGE). Endotoxin: 0.1ng/ug (1EU/ug)
• Formulierung: Supplied as a lyophilized powder from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to 0.1mg/ml. Add 0.1% HSA or BSA for long term storage.