BpiI (BbvII)

Produktübersicht

• Artikelname: BpiI (BbvII)
• Artikelnummer: USB-B2640
• Hersteller Artikelnummer: B2640
• Alternativnummer: USB-B2640-200,USB-B2640-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G A A G A C (N)2-3 3-C T T C T G (N)6 -5 Supplied With: R1625: Restriction Enzyme Buffer A, 10X, Dilute to 1X. 1X buffer compostion is 33mM Tris-acetate pH 7.9 at 37C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-02: Restriction Enzyme Buffer C, 10X, Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 50mM sodium chloride, 0.1mg/ml BSA. Unit Definition: One unit is defined as the amount of BpiI required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of reaction buffer. Diluent Buffer: 10mM Tris-HCl, pH 7.4 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Enzyme Properties: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BpiI. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double-stranded labeled oligonucleotide occurred duringincubation with 10 units of Bpil for 4 hours. Blue/White Cloning Assay: The B/W assay was relpaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Methylation Effects: Dam, Dcm, EcoKl: never overlaps - no effect CpG: may overlap - no effect EcoBl: may overlap - effect not determined Stability during Prolonged Incubation: A minimum of 0.3units of BpiI is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: BpiI is inactivated by incubation at 65C for 20 minutes. Digestion of Agarose-embedded DNA: A minimum of 5units of BpiI is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 24 PhiX174: 3 pBR322: 3 pUC57: 0 pUC18/19: 0 pTZ19R/U: 0 M13mp18/19: 0 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.