Bpl I

Produktübersicht

• Artikelname: Bpl I
• Artikelnummer: USB-B2645
• Hersteller Artikelnummer: B2645
• Alternativnummer: USB-B2645-100,USB-B2645-500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5- 8(N) G A G (N)5 C T C (N)13 -3 3- 13(N) C T C (N)5 G A G (N) 8 -5 BplI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 100-fold increase of BplI activity. The cleavage of DNA by BplI is never complete. Concentration: 5 units/ul Unit Definition: One unit is defined as the amount of Bpl I required to digest 1ug of lambda DNA-Xhol in 1 hour at 37C in 50ul of assay buffer (a maximal cleavage level is achieved at which no change in the fragmentation pattern is observed with further increase of enzyme). Storage Buffer: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Buffers Supplied: R1625 Restriction Enzyme Buffer A, 10X: 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA B2645-01: SAM 0.05mM S-adenosylmethionine Stability during Prolonged Incubation: A minimum of 0.3 units of Bpl I is required for complete digestion of 1ug of DNA in 16 hours at 37C. Thermal Inactivation: Bpl I is inactivated by incubation at 65C for 20min. Methylation Effect: DAM, DCM, EcoKl, EcoBl: never overlaps - no effect CpG: may overlap - no effect Number of Recognition Sites in DNA: Lambda: 1 PhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U: 0 Digestion of Agarose-embedded DNA: A minimum of 10 units of the Bpl I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with BplI. Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities with sensitivity that is higher thab L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Bpl I for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.