| 5-C C T N A G C-3 3-G G A N T C G-5 Concentration: 5u/ul Source: E.coli that carries the cloned bpu10IR gene from Bacillus pumilus RFL10 Buffer: 10mM Bis-Tris Propane-HCl (pH 6.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. |