Bpu1102I (EspI)

Produktübersicht

• Artikelname: Bpu1102I (EspI)
• Artikelnummer: USB-B2650
• Hersteller Artikelnummer: B2650
• Alternativnummer: USB-B2650-200,USB-B2650-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G C T N A G C-3 3-C G A N T C G-5 Source: Bacillus pumilus RFL1102 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Concentration: 10 units/ul R1625 Restriction Enzyme Buffer A, 10X,: 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Double Digests: R1625 Restriction Enzyme Buffer A, 10X (Catalog No: R1625) is provided to simplify buffer selection for double digests. Y+/Tangle Buffer is supplied as a liquid in 33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Incubate at 37C. Dilution/Storage Buffer:: 10mM Tris-HCl, pH 7.4 at 25C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bpu1102I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bpu1102I, more than 80% of the DNA fragments can be ligated in 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5-termini concentration of 0.2uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Enzyme Activity: Methylation Effect: Bpu1102I cuts GCTNAGm5C. Stability during Prolonged Incubation: A minimum of 0.1unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Compatible Ends: Bpu10I, Eco81I, Hpy3I Number of Recognition Sites in DNA: Lambda: 6 PhiX174: 0 M13mp18/19: 0 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 pBluescriptIIKS(-/+): 0 pBluescriptIISK(-/+): 0 pACYC177: 0 pACYC184: 0