Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Artikelnummer:
USB-B2700-01G
Hersteller Artikelnummer:
B2700-01G
Alternativnummer:
USB-B2700-01G-2,USB-B2700-01G-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kD homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and mouse BDNF sequences are identical. Biological Activity: Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1ug/ml. Amino Acid Sequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder. Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions.
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