BseMII

Produktübersicht

• Artikelname: BseMII
• Artikelnummer: USB-B2910-05
• Hersteller Artikelnummer: B2910-05
• Alternativnummer: USB-B2910-05-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C T C A G (N)10 -3 3-G A G T C (N)8 -5 Concentration: 1u/ul Source: Bacillus stearothermophilus Isl 15-111 [Buffer] + SAM: [33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA] + 0.01mM S-adenosylmethionine (supplied with 0.5mM solution). Incubate at 55C. (Incubation at 37C results in 30% activity.) Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 64-fold overdigestion (4u/ug lambda DNA x 16 hours) with BseMII. Ligation/Recutting Assay: After 10-fold overdigestion (2u/ug DNA x 5 hours) with BseMII more than 90% of the DNA fragments can be ligated at a 5-termini concentration of 0.5uM. None of these can be recut due to the methylation at the recognition sequence by restriction enzyme. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Stability during Prolonged Incubation: A minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55C. Thermal Inactivation: Enzyme is inactivated by incubation at 80C for 20min. Number of Recognition Sites in DNA: Lambda: 79 PhiX174: 10 M13mp18/19: 23 pBR322: 7 pUC18/19: 5 pUC57: 5 pTZ19R/U: 4 pBluescriptIIKS(-/+): 4 pBluescriptIISK(-/+): 4 pACYC177: 13 pACYC184: 8 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55C in 50ul of assay buffer.