Bsh1285I (McrI)

Produktübersicht

• Artikelname: Bsh1285I (McrI)
• Artikelnummer: USB-B2922-05
• Hersteller Artikelnummer: B2922-05
• Alternativnummer: USB-B2922-05-600
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C G Pu Py C G-3 3-G C Py Pu G C-5 Concentration: 10u/ul Source: Bacillus sphaericus RFL1285 Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 150mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bsh1285I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bsh1285I, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.1uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Methylation Effect: Blocked by CG methylation. Stability during Prolonged Incubation: A minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: CGGC CG-BseNI, Cfr42I CGGC TG-BseNI CGAT CG-PacI, PvuI, SgfI Number of Recognition Sites in DNA: Lambda: 22 PhiX174: 1 M13mp18/19: 4 pBR322: 7 pUC18/19: 5 pUC57: 5 pTZ19R/U: 5 pBluescriptIIKS(-/+): 6 pBluescriptIISK(-/+): 6 pACYC177: 6 pACYC184: 6 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.