Bsp143I (MboI, Sau3AI)

Produktübersicht

• Artikelname: Bsp143I (MboI, Sau3AI)
• Artikelnummer: USB-B2950
• Hersteller Artikelnummer: B2950
• Alternativnummer: USB-B2950-300,USB-B2950-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Description: 5- G A T C-3 3-C T A G -5 Source: Bacillus species RFL143 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of Bsp143I required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer. Diluent Buffer: For short term storage (3-4 weeks)- dilute with 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol. Storage Buffer: For long term storage: 10mM Tris-HCl pH 7.4, 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA, 50% glycerol. Supplied With: R1625-35 Restriction Enzyme Buffer for Bsp143I, 10X: Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 0.02% Triton X-100, 0.1mg/ml BSA. R1625: Restriction Enzyme Buffer A, 10X: Suplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Enzyme Properties: Compatible Ends: BamHI, BcII, BgIII, Psul Methylation Effects: Dam: completely overlaps - no effect Dcm: never overlaps - no effect CpG: may overlaps - blocked EcoKl: never overlaps - no effect EcoBl: may overlap - no effect Stability During Prolonged Incubation: A minimum of 0.1units of Bsp143I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Bsp143I is inactivated by incubation at 65C for 20min. Number of Recognition Sites in DNA: Lambda: 116 M13mp18/19: 7 pBR322: 22 pUC18/19, pUC57, pTZ19R/U: 15 X174: 0 Note: Dpnl, Bsp1431 and Mbol all recognize the same sequence but have different methylation sensitivites and cleavage sites. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Bsp143I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Bsp143I, more than 95% of the digested DNA fragments can be ligated at a 5-termini concentration of 1.2uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Bsp143I for 4 hours. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.