BspTI (AflII)

Produktübersicht

• Artikelname: BspTI (AflII)
• Artikelnummer: USB-B2970
• Hersteller Artikelnummer: B2970
• Alternativnummer: USB-B2970-1000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C T T A A G-3 3-G A A T T C-5 Concentration: 10u/ul Source: Bacillus species RFL1265I Buffer (1X) (For 100% digestion): R1625-03: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: R1625: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or a Storage Buffer. Form: Supplied as a liquid in 10mM Tris-HCl (pH 7.5 at 25C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with BspTI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with BspLI, approximately 80% of the DNA fragments can be ligated at a 5-termini concentration of 0.2uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/PstI and pUC57/Eco32I digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Stability during Prolonged Incubation: A minimum of 0.1units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: SmlI Methylation Effects on Digestion: Dam: never overlaps, no effect Dcm: never overlaps, no effect CpG: never overlaps, no effect EcoKI: never overlaps, no effect EcoBI: never overlaps, no effect Number of Recognition Sites in DNA: Lambda: 3 PhiX174: 2 M13mp18/19: 0 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 pBluescriptIIKS(-/+): 0 pBluescriptIISK(-/+): 0 pACYC177: 0 pACYC184: 0 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dam-in 1 hour at 37C in 50ul of assay buffer.