BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (PE), Clone: [11K3], Mouse, Monoclonal

Produktübersicht

• Artikelname: BTLA (B- and T-lymphocyte Associated Protein, B- and T-lymphocyte Attenuator, BTLA1, CD272, CD272 Antigen, FLJ16065, MGC129743) (PE), Clone: [11K3], Mouse, Monoclonal
• Artikelnummer: USB-B5001-61L
• Hersteller Artikelnummer: B5001-61L
• Alternativnummer: USB-B5001-61L-120
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FC, FLISA
• Immunogen: Recombinant soluble human CD272.

Produktbeschreibung

Human CD272 (BTLA, B and T Lymphocyte Attenuator) is a member of the Immunoglobulin superfamily and has sequence homology to PD-1 and CTLA4. It is expressed on T and B lymphocytes and other hemopoetic lineages. Engagement of this molecule by its ligand, CD270 (HVEM, a TNF superfamily member) can down regulate activated T and B cell responses. CD272 levels on antigen specific CD8+ T cells have been reported to decrease in viral specific, but not melanoma specific activated lines (1). Polymorphism of the CD272 molecule has been linked to rheumatoid arthritis (3). Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells (PBMC) per tube were pelleted and pre incubated 5 minutes with 20ul of human IgG at 300ug/ml, after which they were washed and incubated 45 minutes on ice with 80ul of B5001-61L diluted 1:50 (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. 3. Cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD272 antibody (Catalog B5001-61G). Storage and Stability: May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [11K3]
• Reinheit: Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
• Formulierung: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2 BSA, 15% glycerol, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE).