Calcitonin Receptor Like Receptor, Rat Control Peptide (CRLR)

Produktübersicht

• Artikelname: Calcitonin Receptor Like Receptor, Rat Control Peptide (CRLR)
• Artikelnummer: USB-C0115-80
• Hersteller Artikelnummer: C0115-80
• Alternativnummer: USB-C0115-80-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

Control Peptide for C0115-90 (antiserum) and C0115-85 (affinity purified) The calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family B which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa, rat/mouse 463aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175aa, rat 182aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ul C0115-90 (antiserum) or per 1ug C0115-85 (affinity purified antibody). ELISA: 50-100ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide