Calmodulin Binding Protein Epitope Tag (CBP-Tag) (HRP), Rabbit

Produktübersicht

• Artikelname: Calmodulin Binding Protein Epitope Tag (CBP-Tag) (HRP), Rabbit
• Artikelnummer: USB-C1035-09-HRP
• Hersteller Artikelnummer: C1035-09-HRP
• Alternativnummer: USB-C1035-09-HRP-100
• Hersteller: US Biological
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Immunogen: A synthetic peptide, MKRRWKKNFIAVSAANRFKKISSSGAL corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors (KLH).

Produktbeschreibung

Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000. Detects a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli. Lysates from E. coli transformed with a plasmid encoding a GST-CBP, calmodulin binding protein epitope tagged. Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-09. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Produkteigenschaften

• Reinheit: Purified by affinity chromatography.
• Formulierung: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).