Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2, Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-
Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2, Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-
Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2, Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-
Artikelnummer:
USB-C1035-25B6
Hersteller Artikelnummer:
C1035-25B6
Alternativnummer:
USB-C1035-25B6-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
WB
Immunogen:
Synthetic peptide surrounding Val184 of human CaMKII-alpha.
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
