Control Peptide for C1078-01, Capsaicin Receptor, polyclonal antibody. Nociception, the process of detecting noxious chemical, mechanical, or thermal stimuli, occurs predominantly at the peripheral terminal neurons known as polymodal nociceptors. Nociceptors transduce noxious stimuli into membrane depolarization that triggers action potential, conducts the action potential from the sensory sites to the synapses in the CNS, and conversion of action potentials invokes a perception of pain, discomfort, and appropriate mechanical/physical protective reflexes. At the molecular level, nociception is carried out by ion channels or receptors. Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel that exchanges ~10 Ca+ ions for every Na+ ion resulting into neuronal membrane depolarization and elevated intracellular Ca+ levels. Over-expression of VR1 caused cell death because of continuos influx of Ca+ ions. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains. The cytoplasmic, N-terminal hydrophilic segment (432aa) contains a relatively proline rich region followed by three ankyrin-repeat domains. Structurally, VR1 closely resembles putative store-operated channels (SOC) similar to Drosophila retinal proteins TRP (transient receptor protein channel) and TRPL. However, VR1 does not appear to be a functional SOC. VR1 expression is limited to small to medium diameter primary sensory neurons. Source: Synthetic peptide (15aa) of rat VR1, C-terminal, Cytoplasmic. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
3
Reinheit:
Purified
Formulierung:
Supplied as a lyophilized pwder from PBS, pH7.5.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
