Carbonyl reductase 3(CBR3) is one of several monomeric NADPH-dependent oxidoreductases. This protein catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It also contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. Some studies suggest that it mediates 9-cis-retinoic acid-induced cytostatis and is a potential prognostic marker for oral malignancy. Source: Recombinant human CBR3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Molecular Weight: 33kD(297aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET PVYLALLPPD ATEPQGQLVH DKVVQNW Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: For long-term storage, aliquot and store at -20C/-70C. Aliquots are stable for at least 6 months at -20C/-70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
