| Carboxypeptidase B (CPB) catalyzes hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-teminal position in polypeptides. It occurs as a zymogen in the pancreas of most vertebrates. Synonyms: EC=3.4.17.2, Porcine pancreas, Protaminase, Peptidyl-L-Lysine [L-Arginine] hydrolase CAS No: 9025-24-5 Molecular Weight: 34.5kD Source: Porcine pancreas Appearance: Chromatographically purified and supplied as a solution in 0.1M sodium chloride. Purity: Chromatographically purified Activity: 170 units/mg protein Unit Definition: 1 unit hydrolyzes 1 micromole of hippuryl-L-arginine per minute at 25C, pH 7.65. Quality Control: SDS-PAGE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
