Carnitine Palmitoyl Transferase 1, Liver, Mouse, Control Peptide (CPT1-L)

Produktübersicht

• Artikelname: Carnitine Palmitoyl Transferase 1, Liver, Mouse, Control Peptide (CPT1-L)
• Artikelnummer: USB-C1385-31
• Hersteller Artikelnummer: C1385-31
• Alternativnummer: USB-C1385-31-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

A 13-aa peptide sequence mapping near the C-terminus of mouse CPT1-L (1). In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-1 liver or CPT1A or LCPT-1 (mouse 764-aa, rat 773-aa, human 773-aa, ~88-kda, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial contact sites. It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation. It is predominantly expressed in kidney, liver and in trace amounts in heart. The muscle isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772-aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain. The aa sequences of the two isoforms are ~61% identical. Applications: Suitable for use in ELISA or Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml per well Antibody Blocking: 5-10ug Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide