Synthetic peptide corresponding to 13aa near the C-terminus of mouse CPT1-M conjugated to KLH. Swiss/UniProt Accession: Q63704. Species Sequence Homology: rat, 92%, human, 69%.
In cells and organisms, the excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1). Activities of ACC-1 and ACC-2 are regulated by their phosphorylation by 5-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch, represses the ACC-1 gene-expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Cytosolic LCFA-CoA is converted into acyl-carnitine at mitochondrial membrane surface by carnitine palmitoyl transferase-1 (CPT-1) followed by the reconversion of the latter into LCFA-CoA by CPT-2 in the mitochondrial matrix. Mitochondrial oxidation of LC-FCA is initiated by the sequential action of CPT-1, which is located in the outer membrane, and CPT-2, which is located in the inner membrane together with a carnitine-acylcarnitine translocase. CPT-1 liver or CPT1A or LCPT-1 (mouse 764aa, rat 773aa, human 773aa, ~88kD, chromosome 11q13) is malonyl-CoA-sensitive enzyme localized on the outer surface of mitochondrial contact sites. It catalyzes the conversion of long-chain acyl-CoA into acyl-carnitine, committing the acyl moiety to intramitochondrial oxidation. It is predominantly expressed in kidney, liver and in trace amounts in heart. The muscle isoform CPT1B or CPT1M or MCPT-1 (mouse/rat/human 772aa, chromosome 22q13.3) is found in heart, skeletal muscle, adipose tissue and brain. The amino acid sequences of the two isoforms are ~61% identical. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000. Coat plates with 50-100ng of C1385-48: Carnitine Palmitoyl Transferase 1, Muscle, Mouse, Control Peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
