Cathepsin L (MEP, CTSL, CTSL1), Clone: [9G334], Mouse, Monoclonal
Artikelnummer:
USB-C2097-70F
Hersteller Artikelnummer:
C2097-70F
Alternativnummer:
USB-C2097-70F-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Isolated from human lung cancer cell line EPLC32M1. The epitope is within the sequence GYGFEST (aa265-271 in procathepsin L and aa169-175 in the mature cathepsin L molecule).
Cathepsin L is a cystein protease. It is important for the overall degradation of proteins in lysosomes. Cathepsin L substrates include collagen, elastin and alpha 1 protease inhibitor. The protein is initially expressed as procathepsin L (42kD) and is proteolytically processed into 30kD and 21kD forms. This antibody recognizes both precursor and mature forms. Applications: Suitable for use in Immunohistochemistry and Western Blot . Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (formalin/acetone fixed tissues): 4ug/ml Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
