Cathepsin L (Cathepsin L heavy chain, Cathepsin L light chain, CATL, CTSL, Major excreted protein, MEP), IgG1, Clone: [8J540], Mouse, Monoclonal
Artikelnummer:
USB-C2097-70J
Hersteller Artikelnummer:
C2097-70J
Alternativnummer:
USB-C2097-70J-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ELISA, IHC, WB
Immunogen:
Purified full length native protein (Human lung cancer line EPLC 32 M1). Epitope: Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). Cellular Localization: Lysosomes.
Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Functional Studies. Other applications not tested. Recommended Dilutions: ELISA: 1:200 Immunohistochemistry: frozen sections Functional Studies: Suppresses the growth of malignant myeloma cells. Optimal dilutions to be determined by the researcher. Positive Control: Whole cell extract from cultured mink lung cell line. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
