Phosphorylated proteins from chick embryo fibroblast.
Caveolin describes a group of 22-24kD integral membrane proteins that are localized to caveolae on the plasma membrane. Caveolae are specialized flask-shaped invaginations of the plasma membrane that are thought to exist in all cell types, but are most prominent in adipocytes, endothelial cells, muscle cells and fibroblasts. Caveolae are thought to play a role in cell signaling and in transcytosis across the plasma membrane. There are three groups of caveolin proteins (caveolin-1, -2 and -3). The gene for caveolin-1 encodes both -1 and -2. Caveolin-3 is expressed primarily in muscle cells. Although caveolin localizes to the plasma membrane, it is insoluble in non-ionic detergents and is believed to be a structural component involved in caveolae assembly. In addition to its structural role, caveolin binds to cholesterol and to G-alpha subunits of heterotrimeric G-proteins. Originally identified with anti-phosphotyrosine antibodies, caveolin is phosphorylated on tyrosine by the Src tyrosine kinase. Phosphorylated caveolin can be detected in v-Src transformed cells, and in cells stimulated with insulin. However, the effect of tyrosine phosphorylation on caveolin function remains to be determined. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 2-4ug/ml Western Blot: 0.5-2ug/ml Immunoprecipitation: 2-5ug/IP Optimal dilutions to be determined by the researcher. Positive control: Human fibroblasts cell lysates Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[3G69]
Isotyp:
IgG1
Reinheit:
Highly purified
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
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