CD4, Recombinant, Human (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4
Artikelnummer:
USB-C2255-27G
Hersteller Artikelnummer:
C2255-27G
Alternativnummer:
USB-C2255-27G-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western Blot. Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs. Applications: Suitable for use in ELISA, Neutralization, Drug Screening and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s). Western Blot: 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s). Neutralization: 1-10ug/ml of the HIV-1 infected culture. Drug screening assays: 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using H6003-34G1, HIV-1 gp120 (HRP) or directly using H6003-07B1 HIV-1 env gp120, Strain IIIB, Recombinant (HRP) Optimal dilution determined by the researcher Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molekulargewicht:
55
Reinheit:
Purified by immunoaffinity and gel exclusion chromatography to ~95% purity as determined by SDS PAGE, reduced.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
