CD9 is a 24-27kD transmembrane protein which is a member of the tetraspanin family. It is thought that the main role of tetraspanins is to form signal transducing complexes with integrins at the cell surface, and for this reason CD9 has multiple functions in cell adhesion and motility, platelet activation, gamete fusion and cell proliferation. CD9 has a functional role as a tumor metastatic suppressor and is expressed in melanomas, and colon, lung and breast carcinomas. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat-1:200, 10ul labels 10e6 cells in 100ul Western Blot: Detects a band at ~25kD in HeLa cell lysates Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
