CD9 is a 24-27kD transmembrane protein which is a member of the tetraspanin family. It is thought that the main role of tetraspanins is to form signal transducing complexes with integrins at the cell surface, and for this reason CD9 has multiple functions in cell adhesion and motility, platelet activation, gamete fusion and cell proliferation. CD9 has a functional role as a tumor metastatic suppressor and is expressed in melanomas, and colon, lung and breast carcinomas. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
