Human WM-1 (Waldenströms macroglobulinemia) cell line.
Human CD39 is found on most mature B cells, activated NK cells and activated T cells. CD39 has homology to the N- myc family of proteins and has been cloned. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml (80ul) using 5x10e5 ficoll prepared huma peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated for about 5 minutes with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of C2391-01L (5ug/ml). 2. Cells were washed twice and incubated with reagent IgG goat anti-mouse (FITC), after which they were washed three times, fixed and analyzed by FACS. 3. A 15% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[7H171]
Isotyp:
IgG1
Reinheit:
Purified by Protein A affinity chromatography.
Formulierung:
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
