CD45R is an isoform of CD45, also known as B220. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker, however, CD19 may be more appropriate for B cell specificity. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 2ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[RA3-6B2]
Reinheit:
Purified by affinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
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